A new method for identifying the origins of simple and complex hopanoids in sedimentary materials using stable isotope labelling with (CH4)-C-13 and compound specific stable isotope analyses

Authors
Crossman, ZM McNamara, N Parekh, N Ineson, P Evershed, RP
Citation
Zm. Crossman et al., A new method for identifying the origins of simple and complex hopanoids in sedimentary materials using stable isotope labelling with (CH4)-C-13 and compound specific stable isotope analyses, ORG GEOCHEM, 32(2), 2001, pp. 359-364
Citations number
16
Categorie Soggetti
Earth Sciences
Journal title
ORGANIC GEOCHEMISTRY
ISSN journal
01466380 → ACNP
Volume
32
Issue
2
Year of publication
2001
Pages
359 - 364
Database
ISI
SICI code
0146-6380(2001)32:2<359:ANMFIT>2.0.ZU;2-W
Abstract
Forest soils were taken and incubated with C-13-labelled methane. The lipid s from these soils were isolated using a Bligh Dyer extraction and fraction ated. Simple hopanoids were present in the neutral fraction, while periodic acid/sodium borohydride treatment of the total lipid extract released prim ary alcohols from polyfunctionalised bacteriohopane derivatives. All hopano ids were analysed by GC, GC/MS and GC/C/IRMS. Specific hopanoids, namely: d iploptene, 17 beta (H), 21 beta (H)-homohopanol and 17 beta (H), 21 beta (H )-bishomohopanol produced by methane oxidising bacteria were significantly enriched in C-13 whilst others showed no enrichment. (C) 2001 Elsevier Scie nce Ltd. All rights reserved.