Membrane- and cell wall-associated heat shock proteins in two genotypes ofbarley seedlings

The heat-shock (HS) response of two genotypes (cv. Onice winter type, and c v. Georgie, spring type) of barley (Hordeum vulgare L.) was compared. Prote in synthesis was markedly reduced in cv. Onice was higher than in cv. Georg ie. The pattern of cytosolic, membrane and cell wall HSPs was analysed by S DS-PAGE in coleoptiles and roots of the two genotypes. Differences and simi larities in coleoptiles and roots of the same genotype and between the two genotypes were observed. In roots of the genotype Onice, LMW and HMW HSPs i solated from the cytosol, membranes and cell walls were resolved into a div erse array of polypeptides by two-dimensional gel electrophoresis. Present results confirm the de novo synthesis of cytosolic and membrane HSPs, and d emonstrate, for the first time, their presence in cell walls.