Rs. Plumb et al., Determination of 4-hydroxytamoxifen in mouse plasma in the pg/mL range by gradient capillary liquid chromatography/tandem mass spectrometry, RAP C MASS, 15(4), 2001, pp. 297-303
Capillary high-performance liquid chromatography (HPLC; 300 mum i.d.) coupl
ed to tandem mass spectrometry has been used to determine the concentration
of 4-hydroxytamoxifen in mouse plasma in the pg/mL range following the adm
inistration of Tamoxifen. A limit of quantification (LOQ) of 100 pg/mL was
achieved using only 25 muL of plasma. The on-column sensitivity was determi
ned to be 100 fg. The column performance was determined isocratically befor
e and after the assay and showed only a 15% reduction in performance after
70 injections of plasma extract. No significant peak band broadening was ob
served due to the mass spectrometer interface using a standard Turbolonspra
y source. Copyright (C) 2001 John Wiley & Sons, Ltd.