Ultrafiltration of demethylchlortetracycline industrial fermentation broths

Citation
A. Morao et al., Ultrafiltration of demethylchlortetracycline industrial fermentation broths, SEP PURIF T, 22-3(1-3), 2001, pp. 459-466
Citations number
6
Categorie Soggetti
Chemical Engineering
Journal title
SEPARATION AND PURIFICATION TECHNOLOGY
ISSN journal
13835866 → ACNP
Volume
22-3
Issue
1-3
Year of publication
2001
Pages
459 - 466
Database
ISI
SICI code
1383-5866(20010301)22-3:1-3<459:UODIFB>2.0.ZU;2-G
Abstract
The ultrafiltration of fermentation broths of demethylchlortetracycline (DM CT) produced at an industrial scale at CIPAN SA, has been carried out, usin g two ultrafiltration systems of different module geometries: a tubular mod ule (B1 from Paterson Candy International Ltd.) and a plate-and-frame modul e (LabStak M20 from Danish Separation Systems). A PVDF and a fluoro polymer ultrafiltration membranes both with a MWCO of 100 KD were used. Although t he highest permeate fluxes were obtained with plate-and-frame geometry, ser ious problems arose in cleaning the whole system, due to accumulation of so lids inside the module as the broths under study are not homogeneous and co ntain oil-mycelium aggregates of macroscopic dimensions that cover a wide r ange of particle size (fi-om less than 100 mum to about 2 mm). The results obtained for the ultrafiltration operation are also compared with those obt ained for the industrial filtration using rotary vacuum filters. The same y ields are achieved for about the same dilution, but ultrafiltration has the advantage of not requiring filter aid and flocculants as the conventional filtration process does. The filtrate quality is also another advantage of the membrane process, as the permeates are completely free of suspended sol ids. A rejection to demethylchlortetracycline related products (namely the undesirable isomer epi-DMCT) by the two membranes under study higher than t hat to DMCT is observed as well. (C) 2001 Elsevier Science B.V. All rights reserved.