DIRECT ANALYSIS OF PROTEIN MIXTURES BY TANDEM MASS-SPECTROMETRY

Authors
YATES JR MCCORMACK AL SCHIELTZ D CARMACK E LINK A
Citation
Jr. Yates et al., DIRECT ANALYSIS OF PROTEIN MIXTURES BY TANDEM MASS-SPECTROMETRY, Journal of protein chemistry, 16(5), 1997, pp. 495-497
Citations number
13
Categorie Soggetti
Biology
Journal title
Journal of protein chemistry → ACNP
ISSN journal
02778033
Volume
16
Issue
5
Year of publication
1997
Pages
495 - 497
Database
ISI
SICI code
0277-8033(1997)16:5<495:DAOPMB>2.0.ZU;2-N
Abstract
Methods to identify proteins contained in mixtures are described. The approach uses microcolumn liquid chromatography and automated tandem m ass spectrometry in conjunction with protein and nucleotide database s earching algorithms. This approach is applied to the identification of proteins obtained by immunoprecipitation reactions, interaction with a GST protein fusion products and interaction with a macromolecular co mplex.