EFFECTS OF FRUCTOSE-1,6-BISPHOSPHATE ON MICROSPHERE-INDUCED CEREBRAL-ISCHEMIA IN THE RAT

Fructose-1,6-bisphosphate has been shown to exert beneficial effects i n different experimental models of cerebral ischemia. In view of this evidence, we have determined whether the compound protects the brain d uring microsphere-induced ischemia. One thousand two hundred microsphe res were injected into female rats through a catheter inserted into th e right common carotid artery and, 15 minutes and again 24 hours later , we intravenously treated the animals with 333 mg Kg(-1) of fructose- 1,6-bisphosphate. The injection of microspheres produced significant c hanges in the rats' gross behavior, in their performance in the beam w alking test, and in their brain lactate concentrations. The treatment with fructose-1,6-bisphosphate significantly attenuated the behavioral alterations induced by microsphere ischemia, but not in reducing brai n accumulation of lactate. Moreover, the compound was shown to amelior ate the blood-brain barrier dysfunction, produced 2 and 4 hours after microsphere injection, evaluated by the Evans blue method. These resul ts suggest that fructose-1,6-bisphosphate possesses salutary propertie s against the damages induced by microsphere ischemia.