D. Leclerc et al., The product of ORF III in cauliflower mosaic virus interacts with the viral coat protein through its C-terminal proline rich domain, VIRUS GENES, 22(2), 2001, pp. 159-165
Using the yeast two-hybrid system, we show that the ORF III product of caul
iflower mosaic virus (pIII) interacts through its C-terminus with the viral
coat protein. The last five amino acids of pIII were essential for the int
eraction and virus infectivity. Deletion of the last three amino acids or t
he mutation F129A decreased the strength of the interaction by 90%. We furt
her show that pIII is closely associated with virus particles found in the
inclusion bodies of infected plants but not in viral particles released fro
m the inclusion bodies by urea treatment.