Sewage effluent discharged to surface water has been shown to contain human
hormones, particularly oestrogens, and synthetic chemicals which may be ab
le to disrupt the endocrine system. Since many surface waters which receive
sewage effluent are subsequently used as drinking water sources, it is imp
ortant to demonstrate that treated drinking water is not contaminated. Oest
rogenic activity in rivers and drinking water in the region of Severn Trent
Water was studied using a combination of bioassay, to integrate exposure o
ver lime, and advanced chemical analysis. There was little or no evidence o
f substances that were oestrogenic, even in waters receiving significant am
ounts of sewage effluent. Oestrogenic activity, as measured in the rainbow
trout vitellogenin assay, was seen at the Tame/Trent confluence but this ac
tivity was relatively weak. There was no activity detected at raw water int
akes and no hormones or substances that are oestrogenic were detected in th
e final drinking water. (C) 2001 Elsevier Science Ltd. All rights reserved.