Nitrogen fertilization rates in cereal production systems are generally det
ermined by subtracting soil test N from a specified N requirement based on
the grain yield goal, which represents the best achievable grain yield in t
he last 4 to 5 yr. If grain yield could be predicted in season, topdress N
rates could be adjusted based on projected N removal. Our study was conduct
ed to determine if the potential grain yield of winter wheat (Triticum aest
ivum L.) could be predicted using in-season spectral measurements collected
between January and March. The normalized difference vegetation index (NDV
I) was determined from reflectance measurements under daytime lighting in t
he red and near-infrared (NIR) regions of the spectra. In-season estimated
yield (EY) was computed using the sum of two postdormancy NDVI measurements
(Jan. and Mar.) divided by the cumulative growing degree days (GDD) from t
he first to second reading. A significant relationship between grain yield
and EY was observed (R-2 = 0.50, P > 0.0001) when combining all nine locati
ons across a 2-yr period. Our estimates of potential grain yield (made in e
arly Mar.) differed from measured grain yield (mid-July) at three sites whe
re yield-altering factors (e.g., late summer rains delayed harvest and incr
eased grain yield loss due to lodging and shattering) were encountered afte
r the final sensing. Evaluating data from six of the nine locations across
a 2-yr period, EY values explained 83% of the variability in measured grain
yield. Use of EY may assist in refining in-season application of fertilize
r N based on predicted potential grain yield.