We have developed porous polymer monoliths (PPMs) that are versatile and ro
bust reversed-phase chromatography media. The PPMs are cast-to-shape, UV-cu
red polymers that form uniform packings within pretreated glass capillaries
and fused-silica chips,No applied pressure is ever needed to flush the PPM
s since they support electroosmotic flow as cast. Such characteristics make
the PPMs useful for chip-based devices: Our results show efficiencies grea
ter than or equal to 150 000 plates/m for both capillary and chip-based sep
arations of polycpclic aromatic hydrocarbons. By changing the monomers, the
hydrophobicity of the polymers, and the direction of the electroosmotic no
w can be altered without degrading chromatographic performance. We describe
here the development of these acrylate-based materials along with both phy
sical and chromatographic characterization.