M. Elasri et al., Acyl-homoserine lactone production is more common among plant-associated Pseudomonas spp. than among soilborne Pseudomonas spp., APPL ENVIR, 67(3), 2001, pp. 1198-1209
A total of 137 soilborne and plant-associated bacterial strains belonging t
o different Pseudomonas species were tested for their ability to synthesize
N-acyl-homoserine lactones (NAHL). Fifty-four strains synthesized NAHL. In
terestingly, NAHL production appears to be more common among plant-associat
ed than among soilborne Pseudomonas spp. Indeed, 40% of the analyzed Pseudo
monas syringae strains produced NAHL which were identified most often as th
e short-chain NAHL, N-hexanoyl-L-homoserine lactone, N-(3-oxo-hexanoyl)-ham
oserine lactone, and N-(3-oxo-octanoyl)-L-homoserine lactone (no absolute c
orrelation between genomospecies of P. syringae and their ability to produc
e NAHL could be found). Six strains of fluorescent pseudomonads, belonging
to the species P. chlororaphis, P. fluorescens, and P. putida, isolated fro
m the plant rhizosphere produced different types of NAHL. In contrast, none
of the strains isolated from soil samples were shown to produce NAHL. The
gene encoding the NAHL synthase in P. syringae pv. maculicola was isolated
by complementation of an NAHL-deficient Chromobacterium mutant. Sequence an
alysis revealed the existence of a luxI homologue that we named psmI. This
gene is sufficient to confer NAHL synthesis upon its bacterial host and has
strong homology to psyI and ahlI, two genes involved in NAHL production in
P. syringae pv. tabaci and P. syringae pu. syringae, respectively. We iden
tified another open reading frame that we termed psmR, transcribed converge
ntly in relation to psmI and partly overlapping psmI; this gene encodes a p
utative LuxR regultory protein. This gene organization, with luxI and luxR
homologues facing each other and overlapping, has been found so far only in
the enteric bacteria Erwinia and Pantoea and in the related species P. syr
ingae pv. tabaci.