Sm. An et al., Simultaneous measurement of denitrification and nitrogen fixation using isotope pairing with membrane inlet mass spectrometry analysis, APPL ENVIR, 67(3), 2001, pp. 1171-1178
A method for estimating denitrification and nitrogen fixation simultaneousl
y in coastal sediments was developed. An isotope-pairing technique was appl
ied to dissolved gas measurements with a membrane inlet mass spectrometer (
MIMS). The relative fluxes of three N-2 gas species (N-28(2), N-29(2), and
N-30(2)) were monitored during incubation experiments after the addition of
(NO3-)-N-15. Formulas were developed to estimate the production (denitrifi
cation) and consumption (N-2 fixation) of N-2 gas from the fluxes of the di
fferent isotopic forms of N-2. Proportions of the three isotopic forms prod
uced from (NO3-)-N-15 and (NO3-)-N-14 agreed with expectations in a sedimen
t slurry incubation experiment designed to optimize conditions for denitrif
ication. Nitrogen fixation rates from an algal mat measured with intact sed
iment cores ranged from 32 to 390 mug-atoms of N m(-2) h(-1). They were enh
anced by light and organic matter enrichment. In this environment of high n
itrogen fixation, low N-2 production rates due to denitrification could be
separated from high N-2 consumption rates due to nitrogen fixation. Denitri
fication and nitrogen fixation rates were estimated in April 2000 on sedime
nts from a Texas sea grass bed (Laguna Madre). Denitrification rates (avera
ge, 20 mug-atoms of N m(-2) h(-1)) were lower than nitrogen fixation rates
(average, 60 mug-atoms of N m(-2) h(-1)). The developed method benefits fro
m simple and accurate dissolved-gas measurement by the MIMS system. By addi
ng the N-2 isotope capability, it was possible to do isotope-pairing experi
ments,vith the MIMS system.