An improved method for extraction of beta-carotene from Blakeslea trispora

Authors
Roukas, T Mantzouridou, F
Citation
T. Roukas et F. Mantzouridou, An improved method for extraction of beta-carotene from Blakeslea trispora, APPL BIOC B, 90(1), 2001, pp. 37-45
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology","Biochemistry & Biophysics
Journal title
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN journal
02732289 → ACNP
Volume
90
Issue
1
Year of publication
2001
Pages
37 - 45
Database
ISI
SICI code
0273-2289(200101)90:1<37:AIMFEO>2.0.ZU;2-0
Abstract
An improved method for the extraction of -beta carotene from Blakeslea tris pora is described. The fermentation broth was steamed at 121 degreesC for 1 5 min, and the liquid was centrifuged at 5000g for 20 min. beta -Carotene w as removed from the biomass by extraction with absolute ethanol at a ratio of 1:100 at 30 degreesC for 2 h in a rotary shaker incubator at 300 rpm. Th e carotenoid pigment was completely removed from the cells after three repe ated extractions. The removal of beta -carotene from B. trispora was higher during the first stage (75%) whereas in the other stages it was very slow.