Effect of bovine seminal ribonuclease (BS-RNase) on pig's bone marrow cells

The effect of bovine seminal ribonuclease (BS RNase) on bone marrow cells i n miniature pigs was studied. BS RNase at the concentration 20 and 100 mug/ ml preincubated 1 h with bone marrow cells from normal miniature pigs did n ot influence the formation of CFU-GM and BFU-E colonies cultivated in methy lcellulose medium. The bone marrow cells kept in 4 degreesC survived better in the presence of BS RNase than control cells cocultivated with bovine se rum albumin (BSA). There was proved that pig bone marrow cells are able to bind the BS RNase on their surface without any morphological damage. For th e experimental bone marrow transplantation in miniature pigs the whole body irradiation of 8G with a cobalt source was used. Two recipients from seven accepted semi-incompatible bone marrow cells preincubated 2 h with BS RNas e (10mg/5x10(8) cells). No recipient from eight transplanted control animal s accepted semi-incompatible bone marrow cells preincubated with BSA.