POLYCLONAL DNASE ABZYME FORMED BY THE ANT IIDIOTYPIC INFERNAL IMAGE METHOD

The concept of antigen internal image was applied to the production of catalytic antibodies. An antibody raised in rabbits to DNase (Ab1), a cted as a competitive inhibitor of the catalysis, and thus was assumed to contain anti-active site Ab. This Ab1 was used to elicite a polycl onal anti-idiotypic antibody (Ab2). This later exhibited a DNA recogni tion specificity, suggesting the existence of structural internal imag es mimicking the conformation of the active site. Moreover, Ab2 were a ble to hydrolyse DNA, indicating the existence of internal images mimi cking the enzymatic activity of DNase. Consequently, a strategy can th us be considered using the idiotypic way, for the production of abzyme s in the form of internal images of enzyme active sites.