Enhanced green fluorescent protein as a useful tag for rapid identification of homozygous transgenic mice

Authors
Sato, M Watanabe, T Oshida, A Nagashima, A Miyazaki, J Kimura, M
Citation
M. Sato et al., Enhanced green fluorescent protein as a useful tag for rapid identification of homozygous transgenic mice, BIOMOL ENG, 17(3), 2001, pp. 83-85
Citations number
15
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOMOLECULAR ENGINEERING
ISSN journal
13890344 → ACNP
Volume
17
Issue
3
Year of publication
2001
Pages
83 - 85
Database
ISI
SICI code
1389-0344(200103)17:3<83:EGFPAA>2.0.ZU;2-Z
Abstract
We developed a technique that simplifies the process of confirming homozygo us transgenics at preimplantation stages, which are the earliest stages use d in test breeding, using enhanced green fluorescent protein as a tag. All the blastocysts obtained by mating with the combination of Tg/Tg male (homo zygous for transgene) x + / + female exhibited fluorescence. (C) 2001 Elsev ier Science B.V. All rights reserved.