Induction of nitric oxide synthase and subsequent production of nitric oxide not involved in interferon-gamma-induced hyperpermeabiIity of Caco-2 intestinal epithelial monolayers

Authors
Satake, M Watanabe, H Miyamoto, Y Shimizu, M
Citation
M. Satake et al., Induction of nitric oxide synthase and subsequent production of nitric oxide not involved in interferon-gamma-induced hyperpermeabiIity of Caco-2 intestinal epithelial monolayers, BIOS BIOT B, 65(2), 2001, pp. 428-430
Citations number
13
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
65
Issue
2
Year of publication
2001
Pages
428 - 430
Database
ISI
SICI code
0916-8451(200102)65:2<428:IONOSA>2.0.ZU;2-B
Abstract
Caco-2 cell monolayers exposed to 1000 U/ml interferon-gamma (IFN-y) for 6 days elicited inducible nitric oxide synthase (iNOS) expression and increas ed translayer permeability. This iNOS increase was blocked by pyrrolidinedi thiocarbamate (an inhibitor of iNOS induction) but it did not suppress the hyperpermeability response. Furthermore, 2,2 '-(hydroxnitrosohydrazino) bis -ethanamine (a No donor) did not increase monolayer permeability. Therefore , IFN-gamma -induced hyperpermeability is not due to its induction of iNOS activity and resulting increases in NO levels.