Mammal-like striatal functions in Anolis I. Distribution of serotonin receptor subtypes, and absence of striosome and matrix organization

Serotonin (5-HT) 5-HT2A and 5-HT2C receptors are thought to play important roles in the mammalian striatum. As basal ganglia functions in general are thought highly conserved among amniotes, we decided to use in situ autoradi ographic methods to determine the occurrence and distribution of pharmacolo gically mammal-like 5-HT2A and 5-HT2C receptors in the lizard, Anolis carol inensis, with particular attention to the striatum, We also determined the distributions of 5-HT1A, 5-HT1B/D, 5-HT3, and 5-HTuptake receptors for comp arison. All 5-HT receptors examined showed pharmacological binding specific ity, and forebrain binding density distributions that resembled those repor ted for mammals. Anolis 5-HT2A/C and 5-HT1A site distributions were similar in both in vivo and ex vivo binding experiments. 5-HT2A&C receptors occur in both high and low affinity states, the former having preferential affini ty for I-125-(+/-)-2,5-dimethoxy-4-iodo-amphetamine hydrochloride (I-125-DO I). In mammals I-125-DOI binding shows a patchy density distribution in the striatum, being more dense in striosomes than in surrounding matrix. There was no evidence of any such patchy density of I-125-DOI binding in the ano le striatum, however. As a further indication that anoles do not possess a striosome and matrix striatal organization, neither H-3-naloxone binding no r histochemical staining for acetylcholinesterase activity (AChE) were patc hy. AChE did show a band-like striatal distribution, however, similar to th at seen in birds. Copyright (C) 2001 S. Karger AG, Basel.