Me. Rhodes et al., Sexual diergism in rat hypothalamic-pituitary-adrenal axis responses to cholinergic stimulation and antagonism, BRAIN RES B, 54(1), 2001, pp. 101-113
The hypothalamic-pituitary-adrenal (HPA) axis has differential physiologica
l activity in male and female animals (sexual diergism). Central cholinergi
c systems stimulate this endocrine axis. In the present study we investigat
ed muscarinic and nicotinic cholinergic influences on HPA axis activity in
male and female rats by pretreatment with selective cholinergic receptor an
tagonists followed by stimulation with physostigmine (PHYSO), an acetylchol
inesterase inhibitor. Hormonal measures were plasma arginine vasopressin (A
VP), adrenocorticotropic hormone (ACTH), and corticosterone (CORT). Male ra
ts had significantly greater AVP and ACTH responses to PHYSO alone than did
females. Scopolamine (SCOP) enhanced the AVP response to PHYSO to a greate
r extent in males than in females. In contrast, mecamylamine (MEC) enhanced
the AVP response in females but decreased it in males. SCOP potentiated, a
nd MEC inhibited, the stimulatory effect of PHYSO on ACTH in both sexes, bu
t SCOP potentiation was greater in males, and MEC inhibition was greater in
females. Absolute CORT increases following PHYSO were greater in females,
but percent increases over baseline were greater in males. Similar to their
effects on ACTH responses, MEC attenuated, and SCOP enhanced, CORT respons
es to PHYSO. These results suggest that cholinergic receptor subtypes may i
nfluence HPA axis activity differentially in male and female rats. (C) 2001
Elsevier Science Inc.