STIMULATION OF COLLAGEN EXPRESSION AND GLYCOSAMINOGLYCAN SYNTHESIS BYMIDKINE IN HUMAN SKIN FIBROBLASTS

Citation
H. Yamada et al., STIMULATION OF COLLAGEN EXPRESSION AND GLYCOSAMINOGLYCAN SYNTHESIS BYMIDKINE IN HUMAN SKIN FIBROBLASTS, Archives of dermatological research, 289(7), 1997, pp. 429-433
Citations number
39
Categorie Soggetti
Dermatology & Venereal Diseases
ISSN journal
03403696
Volume
289
Issue
7
Year of publication
1997
Pages
429 - 433
Database
ISI
SICI code
0340-3696(1997)289:7<429:SOCEAG>2.0.ZU;2-I
Abstract
Midkine is a retinoid-inducible regulator off cell differentiation and cell growth in developing embryonic tissues, The effects of midkine o n the expression of the extracellular matrix components, collagens and glycosaminoglycans, in dermal fibroblasts were studied, Midkine did n ot alter fibroblast proliferation, Collagen synthesis was stimulated i n a dose- and exposure time-dependent manner and the maximum twofold s timulation was achieved at a concentration of 100 ng/ml for 72 h treat ment, Midkine increased the synthesis of type I and III collagens to t he same extent, Glycosaminoglycan synthesis was also stimulated twofol d, mainly acounted for by an increase in hyaluronan synthesis, Midkine enhanced mRNA levels of alpha(1) (I), alpha(2) (III), alpha(1) (VI) c ollagens, transforming growth factor pi and matrix metalloprotease-2 b ut did not after the mRNA levels of elastin, MMP-1 and tissue inhibito r of metalloproteases-1. These results suggest that midkine is a poten t stimulator of collagen and glycosaminoglycan synthesis and may prove useful in the therapy of delayed wound healing.