This is the first survey of Trichomycetes in Puerto Rico. Various arthropod
s (including millipedes, beetles, larval dipterans, isopods, and crabs) wer
e collected from terrestrial, freshwater and marine habitats, and their gut
fungi identified. The four orders of the class Trichomycetes were represen
ted, including the following genera: Amoebidium, Asellaria, Enterobryus, Ge
nistellospora, Harpella, Leidyomyces, Paramoebidium, Smittium and Stachylin
a. These correspond to 60 % (9/15) of the genera previously reported from t
ropical regions worldwide. Results of this survey are combined with the pre
vious reports of tropical Trichomycetes and summarized as a species list. T
he Puerto Rican genera are discussed, highlighting features of particular i
nterest to those considering future studies on Trichomycetes. We highlight
the potential severe underestimation of the diversity of tropical Trichomyc
etes and suggest that further surveys are warranted, especially with the cu
rrent focus on biodiversity and tropical microfungi.