Separation of Astragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection

Authors
Ganzera, M Bedir, E Calis, I Khan, IA
Citation
M. Ganzera et al., Separation of Astragalus saponins by reversed phase high performance liquid chromatography and evaporative light scattering detection, CHROMATOGR, 53(3-4), 2001, pp. 131-134
Citations number
15
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
53
Issue
3-4
Year of publication
2001
Pages
131 - 134
Database
ISI
SICI code
0009-5893(200102)53:3-4<131:SOASBR>2.0.ZU;2-P
Abstract
A new HPLC method permitted the separation of 13 triterpene glycosides isol ated from different Astragalus species within 40 min. A water / acetonitril e gradient was used as eluent and 5 mum RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector, the main saponins of A. membranaceus could be detected at levels as low as 20.0 mug mL(-1). This method facilitated distinction of different Astragalus species as well as the analyses of market products containing A. membranaceus. The results showed variations from 0.019 to 0.184% in the total saponin content of he market products.