Isocratic separation of erythromycin, related substances and degradation products by liquid chromatography on XTerra RP18

Authors
Chepkwony, HK Dehouck, P Roets, E Hoogmartens, J
Citation
Hk. Chepkwony et al., Isocratic separation of erythromycin, related substances and degradation products by liquid chromatography on XTerra RP18, CHROMATOGR, 53(3-4), 2001, pp. 159-165
Citations number
26
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
53
Issue
3-4
Year of publication
2001
Pages
159 - 165
Database
ISI
SICI code
0009-5893(200102)53:3-4<159:ISOERS>2.0.ZU;2-I
Abstract
A simple, sensitive, selective and robust isocratic LC method is described for the analysis of erythromycin on XTerra RP18 The main component, erythro mycin A is separated from all known related substances and degradation prod ucts. Several unknown impurities are also separated. Acetonitrile - 0.2 M K 2HPO4 pH 7.0 - water,(35:5:60, v/v) was used as a mobile phase at 1.0 mL mi n(-1). UV detection was at 215 nm. The robustness of the method was evaluat ed by a full-factorial experimental design.