Diastereomeric and enantiomeric resolution of methoxytetrahydronaphthalenederivatives, melatonin ligand receptors, by HPLC on amylose chiral stationary phases

Five methoxytetrahydronaphthalene derivatives, new agonist and antagonist l igands for melatonin receptors, were resolved into their diastereomers by a nalytical HPLC methods using derivatized amylose, chiral stationary phases. Separation was by using normal phase methodology with a mobile phase of n- hexane-alcohol (ethanol or 2-propanol) and a silica-based amylose iris-(S)- 1-phenylethylcarbamate (Chiralpak AS), or tris-3,5-dimethylphenylcarbamate (Chiralpak AD). The effects of structural variation in the solutes were not ed. Baseline separation was easily obtained in many cases.