Yj. Cho et al., Production of inulooligosaccharides from chicory extract by endoinulinase from Xanthomonas oryzae No. 5, ENZYME MICR, 28(4-5), 2001, pp. 439-445
Inulooligosaccharides (IOS) production from chicory extract was carried out
using endoinulinase obtained from a new isolate, Xanthomonas oryzae No. 5.
The IOS production from chicory extract was maximum when 50 g/liter of chi
cory extract was utilized as the substrate. As the substrate concentration
increased, the IOS production accordingly decreased probably due to substra
te inhibition. For a comparative study, enzyme reactions were carried out f
rom pure inulin as substrate. Though total IOS contents indicated higher IO
S yield with pure inulin compared to that of chicory extract, the distribut
ion of inulooligosaccharide components between pure inulin and chicory extr
act was not significantly different; i.e. DP5 and higher oligosaccharides a
re major products in case of both chicory extract and pure inulin as substr
ate. A considerable amount of oligofructose (about 30%, w/w), which were or
iginally present in chicory extract, resulted in the change of the enzyme k
inetics. A reaction pH 7 was found to be most suitable for enzyme reaction.
The initial reaction rates increased with increasing enzyme dosage, althou
gh the relative composition of the IOS produced remain unchanged. (C) 2001
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