Pwj. Burnet et al., Electroconvulsive shock increases tachykinin NK1 receptors, but not the encoding mRNA, in rat cortex, EUR J PHARM, 413(2-3), 2001, pp. 213-219
Recent studies have suggested that the substance P (tachykinin NK1) recepto
r may be a pharmacological target for the treatment of mood disorders. Here
, the effects of electroconvulsive shock on tachykinin NK1 receptor gene ex
pression in the rat brain was investigated. Rats received either a single e
lectroconvulsive shock or five shocks on alternate days. Quantitative autor
adiography with [I-125]Bolton Hunter-substance P, and in situ hybridisation
histochemistry, were used to measure tachykinin NK1 receptor-binding site
densities and mRNA abundance, respectively. Densities of tachykinin NK1 rec
eptor-binding sites were significantly increased in the cerebral cortex fol
lowing repeated electroconvulsive shock compared to sham treated animals. D
ensities remained unchanged in the hippocampus, striatum and amygdala. Neit
her single nor repeated electroconvulsive shock altered tachykinin NK1 rece
ptor mRNA in the brain regions examined. Hence, repeated electroconvulsive
shock increases tachykinin NK1 receptors in the rat. brain in a regionally
specific way. Upregulation of receptor-binding sites without a change in mR
NA indicates that translational or post-translational mechanisms underlie t
his process. (C) 2001 Published by Elsevier Science B.V.