T. Lynch et al., POLYMERASE CHAIN REACTION-BASED DETECTION OF TROPHERYMA-WHIPPELII IN CENTRAL-NERVOUS-SYSTEM WHIPPLES-DISEASE, Annals of neurology, 42(1), 1997, pp. 120-124
Whipple's disease of the central nervous system (CNS) may be associate
d with normal intestinal histology as a result of minimal or patchy in
volvement. The diagnosis is difficult and is frequently made post mort
em. We studied 6 patients with clinically suspected CNS Whipple's dise
ase; 2 had oculomasticatory myorhythmia (OMM) fitting criteria for a d
iagnosis of definite CNS Whipple's disease. One of the 2 had duodenal
histology highly suggestive of Whipple's disease; the other 5 patients
had normal duodenal histology DNA was extracted from paraffin-embedde
d duodenal tissues in all patients and frozen pontine tissue in 1. Two
primer pairs (W3F-W4R, W3F-W2R) were used in separate polymerase chai
n reactions (PCRs) to amplify fragments of Tropheryma whippelii 16S rD
NA from these tissue samples. PCR amplicons were detected only in the
duodenal tissues from the 2 patients with OMM. The sequences of these
amplicons were identical to the corresponding region of the previously
published Tropheryma whippelii 16S rDNA sequence. PCR-based assays of
intestinal or brain tissue may be of value for confirming, and possib
ly refuting, a clinical diagnosis of CNS Whipple's disease in a patien
t with any combination of dementia, supranuclear gaze palsy, hypothala
mic manifestations, myoclonus, seizures, ataxia, or OMM, especially wh
en tissue histology is unrevealing.