Differential expression of chitin synthase III and IV mRNAs in ascomata ofTuber borchii Vittad

Citation
R. Balestrini et al., Differential expression of chitin synthase III and IV mRNAs in ascomata ofTuber borchii Vittad, FUNGAL G B, 31(3), 2000, pp. 219-232
Citations number
41
Categorie Soggetti
Plant Sciences",Microbiology
Journal title
FUNGAL GENETICS AND BIOLOGY
ISSN journal
10871845 → ACNP
Volume
31
Issue
3
Year of publication
2000
Pages
219 - 232
Database
ISI
SICI code
1087-1845(200012)31:3<219:DEOCSI>2.0.ZU;2-X
Abstract
Balestrini, R., Mainieri, D., Soragni, E., Garnero, L., Rollino, S., Viotti , A., Ottonello, S,, and Bonfante, P. 2000. Differential expression of chit in synthase III and IV mRNAs in ascomata of Tuber borchii Vittad. Fungal Ge netics and Biology 31, 219-232. A full-length genomic clone encoding a clas s III chitin synthase (CHS) and one DNA fragment corresponding to a class I V CHS were isolated from the mycorrhizal fungus Tuber borchii and used for an extensive expression analysis, together with a previously identified DNA fragment corresponding to a class II CHS, All three Chs mRNAs are constitu tively expressed in vegetative mycelia, regardless of the age, mode of grow th, and proliferation capacity of the hyphae, A strikingly different situat ion was observed in ascomata, where class III and IV, but not class II, mRN As are differentially expressed in a maturation stage-dependent manner and accumulate, respectively, in sporogenic and vegetative hyphae, These data, the first on the expression of distinct Chs mRNAs during fruitbody developm ent, point to the different cellular roles that can be played by distinct c hitin synthases in the differentiation of spores of sexual origin (CHS III) or in ascoma enlargement promoted by the growth of vegetative hyphae (CHS IV), (C) 2000 Academic Press.