INFLUENCE OF SOME PESTICIDES ON ACTIVITY OF 7 SERUM ENZYMES (IN-VITRO)

The purpose of this study was to investigate the effects of Endosulfan [6,7,8,9, 10, 10-hexachloro- 1,5. 5a. 6, 9, 9 a hexahydro- 6, 5, meth ano - 2, 4, 3-benzo (e) dioxathpiepin 3- oxide], Mavrik [alpha-cyano - 3-phenoxybenzyl M -(2 chloro-alpha-alpha-alpha-triflouro-p-tolyl)- D - valinate], 2,4-D (2,4-Dichlorophenoxyacetic acid) and Neoran (isoprop yl -4,4-dibromobenzilate) on serum amylase (EC 3.2.1.1), creatine kina se (CK; EC 2.7.3.2) aspartate amino transferase (AST; EC 2.6.1.1), ala nine aminotransferase (ALT; EC 2..6.1.2), alkaline phosfatase (ALP; EC 3.1.3.1), gamma glutamyl transferase (GGT; EC 2.3.2.2) and lactate de hydrogenase (LDH; EC 1.1.1.27). I-50 values of chemicals caused inhibi tion were determined by means of activity percentage -[I] diagrams. En dosulfan had effect of inhibition only on AST. The I-50 value of chemi cal was determined as 3.13x10(-4) M. Mavrik had effects of inhibition on AST, ALT, ALP and LDH. The values of I-50 were 7.83x10(-4) M, 1.17x 10(-4), 1.23x10(-3) M and 2.23x10(-3) M, respectively. 2,4-D had effec ts of inhibition on ALT, ALP, GGT and LDH. The I-50 values of chemical were 6.97x10(-2) M, 5.05x10(-2) M, 2.35x10(-2) M, 1.07x10(-2) M, resp ectively. On the other hand, Neoran had effects of inhibition of all e nzymes except amylase. I-50 values of this compound on other enzymes ( CK, AST, ALT, ALP, GGT and LDH) were 4.41x10(-4) M, 5.06x10(-3) M, 2.4 0x10(-3) M, 5.23x10(-3) FA,4.4x10(-3) M, 5.97x10(-3) M, respectively.