Extended photoreceptor viability by light stress in the RCS rats but not in the opsin P23H mutant rats

PURPOSE. TO determine the effect of light stress on retinal function and lo ng-term photoreceptor viability in Royal College of Surgeons (RCS) rats and the applicability of the light treatment to the opsin P23H mutant rats. METHODS. RCS rats at postnatal day (P)23 were illuminated with 120 foot-can dles (fc) white light for 10 hours. Photoreceptor survival and basic fibrob last growth factor (bFGF) expression were measured at P60 and P83. Retinal function was evaluated by electroretinography. Opsin P23H transgenic rats w ere treated with light at P28 and analyzed at P70 for photoreceptor viabili ty, ultrastructure, and bFGF expression. RESULTS. Light-treated RCS rats at P60 had four to five rows of nuclei vers us one to two rows in untreated littermates. The average amplitude of the E RG b-wave was 28 muV in treated rats, compared with 6 muV in untreated litt ermates. By P83 there was still significant preservation of the ONL in trea ted rats. Immunoblot analysis showed a high expression of bFGF in the treat ed retinas even 2 months after treatment. Illumination of P23H rats at P28 with 120 fc white light for 10 hours caused substantial photoreceptor cell death, although bFGF expression was upregulated. Lowered illumination dosag es continued to cause photoreceptor damage until levels were reached that n either caused damage nor enhanced survival. CONCLUSIONS. Although light stress promotes photoreceptor survival and func tion in the RCS rat, it elicits death signals in the P23H rats that may not be overcome by survival-promoting factors. Therefore, use of light stress to promote photoreceptor survival should be considered with regard to sensi tivity of the mutation to light damage.