A. Dresel et J. Clos, TRANSCRIPTION OF THE LEISHMANIA-MAJOR HSP70-I GENE LOCUS DOES NOT PROCEED THROUGH THE NONCODING REGION, Experimental parasitology, 86(3), 1997, pp. 206-212
Primary transcripts in kinetoplastid protozoa an generally assumed to
he multicistronic, We have analysed the transcription in the gene locu
s which encodes the 70-kDa heat shock protein by using nuclear nln-on
analysis. We find that RNA synthesis in the Hsp70-1 gene locus either
is terminated or pauses within the intergenic region approximately 250
nt downstream of the polyadenylation site. We therefore propose a dis
continuous mode of transcription in the Hsp70 genes of Leishmania majo
r. (C) 1997 Academic Press.