Genetic analysis of peptidoglycan biosynthesis in mycobacteria: Characterization of a ddlA mutant of Mycobacterium smegmatis

Authors
Belanger, AE Porter, JC Hatfull, GF
Citation
Ae. Belanger et al., Genetic analysis of peptidoglycan biosynthesis in mycobacteria: Characterization of a ddlA mutant of Mycobacterium smegmatis, J BACT, 182(23), 2000, pp. 6854-6856
Citations number
21
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
182
Issue
23
Year of publication
2000
Pages
6854 - 6856
Database
ISI
SICI code
0021-9193(200012)182:23<6854:GAOPBI>2.0.ZU;2-K
Abstract
A temperature-sensitive mutant of Mycobacterium smegmatis was characterized that contains a mutation in ddlA, the gene encoding D-alanine:D-alanine li gase. Enzymatic assays using recombinant proteins and D-cycloserine suscept ibility indicate that the A365V mutation in the SMEG35 DdlA protein causes a reduction in enzymatic activity in vitro and in vivo.