Efficient nitrate removal from wetlands depends on denitrification. Ma
crophytes support denitrification by supplying organic carbon. Organic
carbon available to denitrifying bacteria is released from plant litt
er and from living macrophytes. Macrophytes offer attachment surfaces
for epiphytes, also producing organic matter, and for denitrifying bac
teria. Emergent macrophytes are generally more productive than submerg
ed macrophytes, but submerged macrophytes have more epiphytes and offe
r a larger attachment area in the water column for denitrifying bacter
ia. Emergent and submerged vegetation differ in their seasonal pattern
s of release of organic carbon. We conclude that a mixture of emergent
and submerged macrophytes may be beneficial for nitrogen removal in w
etlands with a surface-flow of nitrate-rich water. The influence of ve
getation on wetland hydraulics must also be considered. A wetland desi
gn with deeper parts favoring submerged macrophytes alternating, along
the water few, with shallower parts covered by emergent macrophytes,
may promote denitrification processes and distribution of water flow.