Protein analysis of an individual Caenorhabditis elegans single-cell embryo by capillary electrophoresis

We present a simple one-dimensional electrophoretic map of the expressed pr oteins in a Caenorhabditis elegans embryo. The embryo was taken from an adu lt nematode, injected into a 50-mum I.D. capillary, and lysed. The proteins were fluorescently labeled and then separated by capillary electrophoresis and detected by laser-induced fluorescence. Over 20 components were resolv ed in the 22-min separation. The dynamic range was outstanding for this sep aration, noise in the baseline was less than 0.01%; the amplitude of the la rgest component. (C) 2001 Elsevier Science B.V. All rights reserved.