We have previously reported that rats with diabetes induced by injecting st
reptozotocin into neonates showed remarkably lower blood glucose, urine vol
ume, and glucosuria after administration of Maitake (Grifola frondosa). In
the present study, we investigated the effects of Maitake on insulin concen
tration, organ weight, serum composition, and islets of Langerhans in strep
tozotocin-induced diabetic rats using the same method. The diabetic rats we
re produced by injecting 80 mg/kg B.W. streptozotocin into 2-d-old neonates
. From the age of 9 wk, the rats were given experimental diets for 100 d. T
he diabetes and control groups were given either diets containing 20% Maita
ke (DM and CM groups) or control diets (D and C groups). During administrat
ion of the experimental diets, we measured body weight, food intake, amount
of feces, and serum insulin concentration at glucose loading. The glucose
tolerance test was performed at the 10th week after the start of the experi
mental diets. The D group had an initial fasting blood glucose of 225+/-49
mg/dL, and a maximum blood glucose of 419 +/- 55 mg/dL at 60 min. In the DM
group, however, the initial fasting blood glucose was 170+/-23 mg/dL, and
the maximum blood glucose was 250+/-41 mg/dL at 15 min. Both values were ma
rkedly lower than those in the D group (p<0.05). The insulin concentration
at 15 min. after glucose loading in the DM group was 41+/-16 <mu>U/mL, whic
h was significantly higher than that in the D group (15+/-7 muU/mL) (p<0.05
). After the 100-d experimental period, blood samples were collected. The f
ructosamine level was significantly lower in the DM group (152+/-21 mmol/L)
than in the D group(185+/-13 mmol/L). The concentration of 1.5-A.G. (1.5-a
nhydro glucitol) was significantly higher in the DM group (9.33+/-2.42 <mu>
g/mL) than in the D group (1.33+/- 0.52 mug/mL). Observation of insulin ant
ibody stain in the Langerhans cells of the pancreas using ABC method showed
a decrease insulin antibody stain in the D group. The cells of the DM grou
p were stained more darkly than those of the D group. From these results, w
e Postulated that the bioactive substances present in Maitake can ameliorat
e the symptoms of diabetes.