Enzymatic alcoholysis of palm kernel oil in n-hexane and SCCO2

The use of biocatalysts in supercritical CO2 (SCCO2) has received widesprea d attention in recent years. Biocatalysts have the advantage of substrate s pecificity under mild reaction conditions and SCCO2 has several advantages over liquid solvents such as high solute diffusivities and low viscosity, w hich can accelerate mass transfer-limited enzymatic reactions. Concerning t he enzymatic alcoholysis of vegetable oils, very little experimental data h ave been reported in the literature. Also. to the best of our knowledge, th ere is no comprehensive study available comparing conventional and supercri tical media for this reaction. In this context, the main objective of this work is to compare enzymatic ethanolysis of palm kernel oil taking place in both SCCO2 and in n-hexane as solvents. For this purpose, a Taguchi experi mental design with two levels and four variables was adopted for each syste m to allow the investigation of the influence of process variables on the r eaction conversion and on the enzyme activity. (C) 2001 Elsevier Science B. V. All rights reserved.