Human Jurkat lymphocytes clones differ in their capacity to support productive human immunodeficiency virus type 1 multiplication

CD4-positive human lymphocytic cell lines are essential tools for the study of human immunodeficiency virus (HIV) replication. Jurkat cells are among cells more frequently used for this purpose. In the current study, various cell clones or cells stocks derived from this cell line were shown to vary substantially in their response to viral infection and in their ability to support productive virus multiplication. The formation of syncytia. the eff ect of Vpu on viral export, and especially the specific infectivity of the viruses released, can vary significantly among independent cell stocks. Thi s suggests that the choice of an adequate cell clone or cell line could be critical while evaluating specific properties of the virus and further stre sses the limitations of tissue culture models. However, these observations also raise the possibility of exploiting these differences among cells to s tudy specific aspects of host-cell interactions contributing to viral multi plication. (C) 2001 Elsevier Science B.V. All rights reserved.