IMMUNOCYTOCHEMICAL AND QUANTITATIVE STUDY OF THE TUNICA ALBUGINEA TESTIS IN YOUNG AND AGING MEN

A light and electron microscope immunohistochemical study of the tunic a albuginea from both young and elderly men was carried out to determi ne the distribution of the cells that contain actin, vimentin and/or d esmin, and to evaluate the possible variations with ageing by means of quantitative studies. Testicular volume and testicular parenchyma vol ume decreased significantly with age whereas the tunica albuginea volu me remained unchanged. These results agree with the scanty quantitativ e changes observed in the testicular connective tissue with age, and t he notion that aoe-related changes in testicular volume are principall y restricted to the seminiferous tubules. Three connective tissue laye rs could be distinguished in the tunica albuginea in both young and el derly men. The middle and inner layers increased in width with age whi le the width of the outer layer decreased. The average width of the tu nica albuginea increased significantly with ageing. The tunica albugin ea of young men and elderly men presented two types of fusiform cells: (1) fibroblast-like cells, which immunoreacted to actin and vimentin, but not to desmin; and (2) myoid cells, which immunoreacted to actin, vimentin and desmin. In both young men and elderly men, the total num ber of desmin-positive cells (myoid cells) was significantly lower tha n that of fibroblasts. However, the total number of desmin-positive ce lls was significantly increased in ageing men. In young testes, desmin -positive cells were more abundant in the outer layer of the tunica al buginea, whereas in elderly men these cells predominated in the middle layer. The increased desmin immunoexpression in the tunica albuginea of ageing men contrasts with the decrease in desmin immunoreaction in other myoid cells of the testis, the peritubular myoid cells, but only in seminiferous tubules that showed severe germ cell depletion. This suggests that changes in intermediate filament immunoexpression in per itubular cells are focalised, and thus, under local control, whereas c hanges in the tunica albuginea cells are generalised and possibly rela ted to factors also affecting the connective tissue in other organs.