J. Minones et al., Orientational changes of amphotericin B in Langmuir monolayers observed byBrewster angle microscopy, LANGMUIR, 17(5), 2001, pp. 1477-1482
The morphology of amphotericin B monolayers spread at the air/water interfa
ce was investigated at the microscopic level with a Brewster angle microsco
pe. The images were recorded simultaneously with the surface pressure (pi)/
area (A) isotherms. The transformation from the domain-like structure to ho
mogeneous solid phase was observed upon monolayer compression. Quantitative
analysis was made from the measurements of relative reflectivity during mo
nolayer compression/decompression. A 3-fold increase in relative monolayer
thickness throughout the compression from the expanded to a condensed state
is observed and related to orientational changes of the monolayer molecule
s from a horizontal to a vertical position.