COMBINATION OF NONISOTOPIC IN-SITU HYBRIDIZATION WITH DETECTION OF ENZYME-ACTIVITY, BROMODEOXYURIDINE INCORPORATION AND IMMUNOHISTOCHEMICALMARKERS

The advent of non-isotopic in situ hybridisation allows the possibilit y to detect the presence of both mRNAs and other markets in cells. We have established conditions for simultaneous analysis of gene expressi on and a variety of other immunohistochemical markers in tissue sectio ns. We report the analysis of expression of a family of transcription factors (Sox genes) in combination with detection of: (1) protein anti gens (using both monoclonal and polyclonal antibodies): (2) bromodeoxy uridine to mark cells which are proliferating; and (3) acetylcholinest erase activity. The approaches we describe, which demonstrate the comp atibility of non-isotopic in situ hybridisation with a range of other treatments, should be generally applicable and open to many variations of probe, antibodies and colour detection systems.