M. Rex et al., COMBINATION OF NONISOTOPIC IN-SITU HYBRIDIZATION WITH DETECTION OF ENZYME-ACTIVITY, BROMODEOXYURIDINE INCORPORATION AND IMMUNOHISTOCHEMICALMARKERS, HISTOCHEM C, 107(6), 1997, pp. 519-523
The advent of non-isotopic in situ hybridisation allows the possibilit
y to detect the presence of both mRNAs and other markets in cells. We
have established conditions for simultaneous analysis of gene expressi
on and a variety of other immunohistochemical markers in tissue sectio
ns. We report the analysis of expression of a family of transcription
factors (Sox genes) in combination with detection of: (1) protein anti
gens (using both monoclonal and polyclonal antibodies): (2) bromodeoxy
uridine to mark cells which are proliferating; and (3) acetylcholinest
erase activity. The approaches we describe, which demonstrate the comp
atibility of non-isotopic in situ hybridisation with a range of other
treatments, should be generally applicable and open to many variations
of probe, antibodies and colour detection systems.