T. Matsui et al., Mycelial growth inhibition of Shiitake (Lentinula edodes) by several terpenoids isolated from Sugi (Cryptomeria japonica) wood, MOKUZAI GAK, 47(1), 2001, pp. 58-62
Methanol extracts of sugi (Cryptomeria japonica D. Don) wood were separated
into toluene-insoluble. hexane-toluene-insoluble. and hexane-toluene-solub
le fractions. Ferruginol, sugiol, sandaracopimarinol, sandaracopimarinal, p
hyllocladanol, and beta -sitosterol were obtained from the hexane-toluene-s
oluble fraction by column chromatography on silica gel. The inhibitory acti
vity of each terpenoid on mycelial growth of shiitake (Lentinula edodes (Be
rk.) Pegler) was examined on a potato dextrose and agar medium at 25 degree
sC. Ferruginol, sugiol, and sandaracopimarinol were found to inhibit the my
celial growth of shiitake at a concentration of 5000 mug cm(-3). The 50% in
hibition concentration of ferruginol was determined at ca. 100 mug cm(-3) a
nd that of sandaracopimarinol at ca. 1000 mug cm(-3). The mycelial growth i
nhibition of shiitake is probably due to a synergistic effect of ferruginol
and sandaracopimarinol, which are the major terpenoids in sugi wood.