Mycelial growth inhibition of Shiitake (Lentinula edodes) by several terpenoids isolated from Sugi (Cryptomeria japonica) wood

Methanol extracts of sugi (Cryptomeria japonica D. Don) wood were separated into toluene-insoluble. hexane-toluene-insoluble. and hexane-toluene-solub le fractions. Ferruginol, sugiol, sandaracopimarinol, sandaracopimarinal, p hyllocladanol, and beta -sitosterol were obtained from the hexane-toluene-s oluble fraction by column chromatography on silica gel. The inhibitory acti vity of each terpenoid on mycelial growth of shiitake (Lentinula edodes (Be rk.) Pegler) was examined on a potato dextrose and agar medium at 25 degree sC. Ferruginol, sugiol, and sandaracopimarinol were found to inhibit the my celial growth of shiitake at a concentration of 5000 mug cm(-3). The 50% in hibition concentration of ferruginol was determined at ca. 100 mug cm(-3) a nd that of sandaracopimarinol at ca. 1000 mug cm(-3). The mycelial growth i nhibition of shiitake is probably due to a synergistic effect of ferruginol and sandaracopimarinol, which are the major terpenoids in sugi wood.