Caspase activation as an apoptotic evidence in the gerbil hippocampal CA1 pyramidal cells following transient forebrain ischemia

To determine whether apoptotic process is involved in the delayed neuronal death in hippocampal CA1 region following forebrain ischemia in gerbils, ti me dependent activation of caspase and DNA fragmentation were evaluated by immune-staining and terminal dUTP nick-end-labeling staining, respectively. After transient forebrain ischemia in gerbils, activation of apoptosis rel ated caspase, including caspase-3, was apparent, and it preceded DNA fragme ntation in CA1 region, These observations suggest that apoptotic process is involved in hippocampal delayed neuronal death. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.