Binding of a porphyrin conjugate of Hoechst 33258 to DNA. I. UV-visible and melting studies detect multiple binding modes to a 12-mer nonself-complementary duplex

Authors
Frau, S Bichenkova, EV Fedorova, OS Lokhov, S Douglas, KT
Citation
S. Frau et al., Binding of a porphyrin conjugate of Hoechst 33258 to DNA. I. UV-visible and melting studies detect multiple binding modes to a 12-mer nonself-complementary duplex, NUCLEOS NUC, 20(1-2), 2001, pp. 131-143
Citations number
29
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
1-2
Year of publication
2001
Pages
131 - 143
Database
ISI
SICI code
1525-7770(2001)20:1-2<131:BOAPCO>2.0.ZU;2-5
Abstract
Relative to ligand-free duplex DNA, the melting temperature of the 1:1 comp lex of the duplex d(CGAATTGTATGC):d(GCATACAATTCG) with the conjugate of Hoe chst 33258 with a des-metalloporphyrin, increased from 42 to 60.5 degreesC indicating strong ligand binding. UV-vis spectrophotometric titration detec ted more than one class of binding site (apparent dissociation constants si milar to0.2 muM for simple noncooperative binding and 1 muM for the simulta neous cooperative mode with Hill coefficient similar to2).