Binding of a desmetallo-porphyrin conjugate of Hoechst 33258 to DNA. III. Strong binding to single-strand oligonucleotides

Authors
Bichenkova, EV Frau, S Fedorova, OS Douglas, KT
Citation
Ev. Bichenkova et al., Binding of a desmetallo-porphyrin conjugate of Hoechst 33258 to DNA. III. Strong binding to single-strand oligonucleotides, NUCLEOS NUC, 20(1-2), 2001, pp. 157-168
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
ISSN journal
15257770 → ACNP
Volume
20
Issue
1-2
Year of publication
2001
Pages
157 - 168
Database
ISI
SICI code
1525-7770(2001)20:1-2<157:BOADCO>2.0.ZU;2-T
Abstract
The binding of the conjugate of Hoechst 33258 with 5,10,15,20-tetrakis (1-m ethyl-4-pyridyl)-21H,23H-porphyrin (PORHOE) to;single-strand DNA has been d etected by UV-vis spectrophotometry and H-1-NMR. The red-shift of porphyrin Soret band with strong hypochromicity indicates that the porphyrin moiety dominates in the interaction of the PORHOE with ssDNA. The affinity constan ts of PORHOE for d(GCATACAATTCG) or d(CGAATTGTATGC) were determined to be > 10(5) M-1, with strong cooperativity.