DEVELOPMENT OF AUTOMATED HIGHLY SENSITIVE ANALYTICAL SYSTEM FOR GUANETHIDINE SULFATE IN SERUM

An automated trace analytical system for Guanethidine sulfate in sera was developed using a liquid chromatograph with column-switching and p ost-column reaction detection systems. Guanethidine sulfate was post-l abeled with ninhydrin in alkaline solution, and the reaction products were monitored with a fluorescence detector. Guanethidine sulfate, an antihypertensive agent, in rat and human sera was analyzed using this automated analyzer. The detection limit of guanethidine sulfate was 1. 0 ng/mL, and the calibration curve was linear from 3.1 ng/mL to 1000 n g/mL with relative standard deviation of less than 3%. The recovery fr om sera was 99.7 +/- 3.9%.