THE INFLUENCE OF BETA-CYCLODEXTRIN ON THE KINETICS OF 1-EN-DEHYDROGENATION OF 6-ALPHA-METHYLHYDROCORTISONE BY ARTHROBACTER-GLOBIFORMIS CELLS

beta-Cyclodextrin changes the kinetic peculiarities of microbial 1-en- dehydrogenation of 6 alpha-methylhydrocortisone: the reaction rate, de gree of conversion and respiratory-chain activity are increased. Respi ratory-chain activity in the presence of beta-cyclodextrin is increase d both in the presence and in the absence of 6 alpha-methylhydrocortis one. A mathematical model is proposed to describe the kinetics of the process. This model suggests the formation of different multi-componen t complexes consisting of inclusion complexes and the functional unit involving the enzyme and the respiratory chain. All the parameters of the model were estimated by data fitting. In the model framework the f ormation of multi-component complexes leads to an increase of the maxi mal reaction rate and to a decrease of substrate inhibition. An approa ch is suggested for optimisation of 6 alpha-methylhydrocortisone 1-en- dehydrogenation in the presence of beta-cyclodextrin. The optimal conc entrations of 6 alpha-methylhydrocortisone and beta-cyclodextrin have been calculated.