Identification of Co-1 anthracnose resistance and linked molecular markersin common bean line A193

Phaseolus vulgaris line A193 has been shown to be widely resistant to Colle totrichum lindemuthianum, including race 1472, one of the most virulent rac es of C. lindemuthianum characterized. Resistance to C. lindemuthianum race 1472 in P. vulgaris line A193 was investigated in segregating F-2 and F-2. 3 populations from a cross between A193 and Flor de Mayo Bajio (a commercia l cultivar highly susceptible to C. lindemuthianum). Resistance to 1472 in A193 was determined to be conditioned by a single dominant gene. Inoculatio n of crosses between A193 and cultivars Michigan Dark Red Kidney and Ferry Marrow with race 1472 suggest that resistance in A193 is conditioned by the Co-1 gene. Inoculation of the cross A193 x Ferry Marrow with C. lindemuthi anum race 2, demonstrated that resistance to race 2 in Ferry Marrow is also conditioned by a single dominant gene and is distinct to resistance in A19 3 or Michigan Dark Red Kidney. Three AFLP markers (ECAG/MACC-1, EACA/MAGA-2 , EAGG/MAAC-8) linked in repulsion to the Co-l locus were identified by scr eening the A193 x Flor de Mayo F-2 population with 314 random amplified pol ymorphic DNA, amplified fragment length polymorphism. and restriction fragm ent length polymorphism markers. The two most closely linked markers should be useful in marker-assisted selection for Co-1.