DETECTION OF 8-HYDROXYDEOXYGUANOSINE, A MARKER OF OXIDATIVE DNA-DAMAGE, IN WHITE BLOOD-CELLS OF WORKERS OCCUPATIONALLY EXPOSED TO STYRENE

Styrene-7,8-oxide (SO), the major in vivo metabolite of styrene, is a genotoxic compound and a potential carcinogenic hazard to occupational ly exposed workers. The aim of the present work was to investigate the ability of styrene exposure to induce formation of 8-hydroxy-2'-deoxy guanosine (8-OHdG) in white blood cells (WBC) of boat builders occupat ionally exposed to styrene. The study of these adducts was conducted t o see if styrene exposure can cause oxidative damage of DNA. The 8-OHd G/10(5) dG ratio from 17 styrene-exposed workers showed significant in creases (mean +/- SD, 2.23 +/- 0.54, median 2.35, P < 0.001) in compar ison to the controls (1.52 +/- 0.45, median 1.50). However, 11 out of 17 workers who were between the ages of 32 and 60 years and had been o ccupationally exposed to styrene for,IO years showed higher 8-OHdG/10( 5) dG ratios (2.31 +/- 0.62, median 2.37) in comparison to 6 workers w ith < 6 years of occupational styrene-exposure (2.11 +/- 0.36, median 2.05; P > 0.05, no significant difference between the two groups of wo rkers). The studies presented here provide an indication that styrene exposure can result in oxidative DNA damage.