Z. Zaman et al., Interaction of a transcriptional repressor with the RNA polymerase II holoenzyme plays a crucial role in repression, P NAS US, 98(5), 2001, pp. 2550-2554
Citations number
47
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
The yeast transcriptional repressor Tup1, tethered to DNA, represses to str
ikingly different degrees transcription elicited by members of two classes
of activators. Repression in both cases is virtually eliminated by mutation
of either member of the cyclin-kinase pair Srb10/11. In contrast, telomeri
c chromatin affects both classes of activators equally, and in neither case
is that repression affected by mutation of Srb10/11. In vitro, Tup1 intera
cts with RNA polymerase II holoenzyme bearing Srb10 as well as with the sep
arated Srb10. These and other findings indicate that at least one aspect of
Tup1's action involves interaction with the RNA polymerase II holoenzyme.