D. Monti et al., RESISTANCE TO APOPTOSIS IN FANCONI ANEMIA - AN EX-VIVO STUDY IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS, FEBS letters, 409(3), 1997, pp. 365-369
Fanconi's anaemia (FA) is a rare autosomal recessive disease character
ised by progressive pancytopoenia, a diverse assortment of congenital
malformations, an increased sensitivity to reactive oxygen species and
a predisposition to the development of malignancies. In the present s
tudy, me assessed the propensity to undergo apoptosis of peripheral bl
ood mononuclear cells (PBMC) from Italian FA patients, Cells were chal
lenged by 2-deoxy-D-ribose (dRib) or TNF-alpha plus cycloheximide as a
gents that induce apoptosis by interfering with cell redox status and
mitochondrial membrane potential (MMP), and PBMC from FA patients resu
lted to be less prone to die than those from healthy subjects. The dec
reased susceptibility of FA cells to undergo apoptosis was also eviden
t when another parameter highly correlated with the apoptotic process,
i.e. MMP, was measured. Moreover, when N-acetylcysteine was added to
dRib-treated PBMC, a strong protection was evident either in PBMC from
control subjects or from FA patients, These data indicate that an alt
eration of unknown nature of the mechanisms favouring apoptosis is pre
sent in freshly collected cells from FA patients, and that such altera
tion could contribute to the pathogenesis of the disease, and particul
arly to the increased susceptibility to cancer. (C) 1997 Federation of
European Biochemical Societies.